[Histonet] Sectioning problems with Fisher Replacement Paraffin

[Bacon, Charles]

Hi Eric,

We use a great product from Polyscientific, called "Infiltrating/Embedding Paraffin Prills" Item Number: c827 https://www.polyrnd.com/product/infiltratingembedding-paraffin-prills/. I am in no way affiliated with this company so this is just a simple Histotech review. As the name suggests, it can actually be used for infiltration in processing and for embedding/microtomy.  I have used it at two different medical centers here in the Western Massachusetts area with great luck. I will tell you that we have Sakura processors and embedding centers. We use Leica RM2255  microtomes, and we do keep our blocks on ice while cutting. I hope this info helps.

Good luck, 

Chuck Bacon, HTL(ASCP)CM 
Supervisor Histology
Baystate Medical Center
361 Whitney Ave., Holyoke, MA 01040
Telephone: 413-322-4786  Fax: 413-322-4790
Charles.Bacon at baystatehealth.org

-----Original Message-----
From: Gilchrist, Eric P [mailto:Eric_Gilchrist at unc.edu] 
Sent: Tuesday, July 06, 2021 9:19 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Sectioning problems with Fisher Replacement Paraffin

Good Morning,

When Fisher stopped producing The TissuePrep 2 Paraffin formulation last year, we were told by the Fisher rep that the new "Histoplast PE" was an equivalent replacement for the TissuePrep 2.  We have had nothing but irritation with this formulation with a magnitude increase in wrinkles and folds. In short, it is NOT an equivalent product.  Does anyone have any recommendations?  Is the "Histoplast LP" any better?

Thanks in advance for your responses.

Eric Gilchrist, Supervisor
UNC Oral and Maxillofacial Pathology Laboratory

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