[Histonet] Bouin's in a spray bottle

Terri Braud tbraud at holyredeemer.com
Tue Feb 9 07:46:20 CST 2021

I have to agree with Bob.  Bouin's should not be used as an ink fixative, especially in a spray bottle.  We use 5% Vinegar, or glacial acetic acid, and it works just fine.
Just my 2 cents - T

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
HNL Labs, Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

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Today's Topics:

   1. Re: Bouin's fixative (Bob Richmond)


Message: 1
Date: Sat, 6 Feb 2021 13:22:22 -0500
From: Bob Richmond <rsrichmond at gmail.com>
To: "Histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Bouin's fixative
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> Bouin's fixative has no business being used in a spray bottle. It's a very
> toxic material that shouldn't be aerosolized.

As Dr. Steve McClain suggests, dilute acetic acid - 3 to 5%, white vinegar,
is quite sufficient for preparing tissue for inking, and isn't toxic and
messy like Bouin's fixative, or flammable like acetone.

Bob Richmond
Samurai Pathologist
Maryville TN


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