[Histonet] Delays in tissue embedding after mouse tissue

Hobbs, Carl carl.hobbs at kcl.ac.uk
Sat Sep 5 03:39:25 CDT 2020

Hi Jamie
I do this often, for he same reason
Ms and rat soft organs ( hollow and solid) leg bones, whole ankles, ears, cube of tissue containing cochlea, chick ribs.....
Once  the delay was 6 months. I allowed the cassettes with tissue in to solidify ( as do you) and then leave them in a labelled box to await embedding.
Sometimes the researcher cannot embed at scheduled time and I would rather fix then process than have fixed tissues sitting in 70% alcohol until they can be processed and embedded on schedule ( sure, it doesn't adversely affect, in my xp, to leave in 70% alc at 4C provided the tissues have been optimally fixed ( meaning in 10% Formalin)

Obviously I strongly discourage any delay, telling them that it is deleterious
Gotta keep them Researchers in order
Best wishes

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London

020 7848 6813

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