[Histonet] Research IMF help

[Porter, Amy]

To all - I am working with NBF fixed paraffin embedded tissue and need to do IMF staining potentially using two different pH retrievals, that are all the same primary host species - I know that if I am doing bright field, I would retrieve, stain for primary 1 with DAB first and the sequentially retrieve and stain for primary 2 with a different chromogen...... any suggestions when working with fluorescence?  Does the first reaction get removed from the retrieval for the second epitope; I am in new territory and could use some recommendations..... TIA.  Amy

Amy S. Porter, HT (ASCP) - Lab Supervisor
Michigan State University
Investigative HistoPathology Lab
567 Wilson Road - Rm 2133
East Lansing, MI  48824
Ph:  517-884-5026
"Life is like riding a bicycle. To keep your balance, you must keep moving." - Albert Einstein