[Histonet] MelanA

[Colleen Forster]

Rhinda,

Which Alkaline Phos detection? How old is the kit?

I have found that the Alk Phos detection  can die before the stated out
date. I use a Biocare platform as well. I have learned to leave the
chromagen 4 degrees until I am ready to stain. I make what I need and
immediately [lace the whole kit back into 4 degrees.

Just one thought.

Colleen Forster HT(ASCP)QIHC
U of MN

On Thu, Oct 22, 2020 at 2:30 PM Rhonda McCormick via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

>  I thought I'd reach out and see if anyone can help with some IHC
> troubleshooting (or know someone who might). Our Melan A that once was
> working on our canine tissue, no longer is.
>
> We are using:
>
> 1:300 dilution of the Leica MelA antibody
>
> BioCare IHC platform
>
> Antigen Retrieval performed in pressure cooker
>
> Alkaline Phosphatase detection
>
>
> We are also using known controls and still... no staining.
>
> We'd appreciate any ideas or recommendations (or even questions that might
> lead to those "lightbulb" moments). Sometimes when you're so mired in the
> problem, it just takes someone else to shed some light on it.
>
>
> Thank you
>
>
> Rhonda
>
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-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
612-626-1930