[Histonet] IHC DAB

[Ana Maluenda]

Hi Kristy,


As the others said, so many things that could be on the way...But making sure your system is based on a HRP enzyme is definitely a good start, as mentioned by Joe.


If you can share details, I might be able to help too.


Welcome to the IHC world!


Cheers,


Ana


Ana Maluenda
Research Assistant/Laboratory Manager
Atherothrombosis and Vascular Biology Laboratory


________________________________
From: Joe Myers <jmyers1 at aol.com>
Sent: Saturday, 2 May 2020 4:49 AM
To: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC DAB

Kristy:
I’m thinking along the same lines as Paula; is it possible that your detection reagent contains only Alkaline Phosphatase  (ALP) as there a reactive enzyme?  If so, a peroxide/DAB solution simply won’t react with it.  Can’t wait to see your protocol, with detailed descriptions of the antigen, pretreatment reagent and ‘labeling’ enzyme.
Cheers,
Joe Myers, M.S.,CT/QIHC(ASCP)



________________________________
From: Kristy Castillo via Histonet

 <histonet at lists.utsouthwestern.edu>
Sent: Friday, May 1, 2020 11:00 AM
To: histonet at lists.utsouthwestern.edu<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] IHC DAB

Hi Histonetters,
We are starting our IHC (fun times), we are having trouble with the DAB lighting up.  Processed for 5 thru 10 minutes and still nothing.  Our permanent red is working just fine.  Any ideas.
Thank you!
Kristy
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