[Histonet] BRAF testing

[John Garratt]

CPQA have performed at least six B-Raf quality assurance challenges over the last few years using TMAs made of 40 tissue cores with known mutational status. Participating labs
have consistently done well and their protocols are available within the reports. In addition to the results there are some photographs for reference. Go to http://cpqa.ca/assessments/Run%20101%20BRAFV600E%20Summary.pdf to see the B-Raf report from last year.

Should you be interested in a clinically based EQA programme for IHC or just wish to know more about our organisation go to www.cpqa.ca
Also, I do have a detailed power point on B-Raf validation, which includes other tissue types and cell lines. Please contact me directly for that if interested. Since we have validated cell lines you may wish to consider using them for working up the antibody. Go to http://www.histocyte.com/ and https://www.statlab.com/truq for cell line information.


John.garratt at cpqa.ca



‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Friday, March 27, 2020 7:05 AM, Cartun, Richard via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> This is a very difficult target to detect. I was told recently by a vendor that one needs to use prolonged, very aggressive antigen retrieval (pressure cooker with high pH buffer) to get consistent results. Also, the type of cancer may influence your success (colon vs. thyroid vs. melanoma). I would be interested in knowing what others' experiences are.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 972-1596 (Office)
> (860) 545-2204 (Fax)
> Richard.cartun at hhchealth.org
>
> -----Original Message-----
> From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Friday, March 27, 2020 8:07 AM
> To: Histo List
> Subject: [Histonet] BRAF testing
>
> This is an email from an external source. USE CAUTION opening attachments or links from unknown senders.
>
> Can anyone send me a block that can be used to validate BRAF V600E antibody. I have tried several cases that should work but our pathologists don't think it is working correctly. I just need a small core sample that is known to be positive. Any help would greatly be appreciated
>
> -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>
> Charles Riley BS HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
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