[Histonet] BRAF testing

[Cartun, Richard]

This is a very difficult target to detect.  I was told recently by a vendor that one needs to use prolonged, very aggressive antigen retrieval (pressure cooker with high pH buffer) to get consistent results.  Also, the type of cancer may influence your success (colon vs. thyroid vs. melanoma).  I would be interested in knowing what others' experiences are.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
Richard.cartun at hhchealth.org

-----Original Message-----
From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, March 27, 2020 8:07 AM
To: Histo List
Subject: [Histonet] BRAF testing

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Can anyone send me a block that can be used to validate BRAF V600E  antibody.  I have tried several cases that should work but our pathologists don't think it is working correctly. I just need a small core sample that is known to be positive. Any help would greatly be appreciated

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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