[Histonet] Mounting media for immunofluorescence slides

Tina Van Meter tina.vanmeter at gmail.com
Fri Mar 6 09:26:59 CST 2020

ProLong Gold - with or without DAPI

On Fri, Mar 6, 2020 at 10:23 AM Martha Ward-Pathology via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> This may be an odd request but I am hoping someone out there can help us
> with this problem.     Our institution is purchasing new digital scanners
> (Olympus 200) to scan all our pathology case slides.     It has come to our
> attention that the mounting media we have all been using for cover slipping
> our direct immunofluorescence slides (skins and renal biopsies using FITC
> IgG, etc. antibodies) is incompatible with the use of these new scanners.
>  We use a hard set mounting media from Vector but it is never quite hard
> enough to prevent any movement of the cover slip, which is apparently
> critical to the scanning process and potentially damaging to the scanner.
>   The techs that are using the scanners are advising us to use a permanent
> mounting media such as Cytoseal 60.     Has anyone encountered this problem
> and was there any downside to using something like Cytoseal 60 on the
> slides?   We plan to test a few control slides to see how they look.
> Thanks in advance for any advice you can give me.
> Martha Ward
> Molecular Diagnostics Lab
> Wake Forest Baptist Health
> Winston-Salem, NC 27157
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