[Histonet] stored in 80% isopropanol

[Betsy Molinari]

Yes, but I plan to put them in my processor starting at 95% ETOH and continue with my regular processing. I am assuming there should be no issues with that correct?

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
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From: Willis, Donna G <Donna.Willis at BSWHealth.org>
Sent: Wednesday, June 3, 2020 5:43 PM
To: Betsy Molinari <BMolinari at texasheart.org>; 'Histonet at lists.utsouthwestern.edu' <histonet at lists.utsouthwestern.edu>
Subject: Re: stored in 80% isopropanol


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80% Isopropanol has 20% water. You will still have water in the tissue.

Donna Willis
Anatomic Pathology Manager
Baylor University Medical Center
Baylor Scott&White Health
214-820-2465 office
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From: Betsy Molinari via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>>
Sent: Wednesday, June 3, 2020 1:39:37 PM
To: 'Histonet at lists.utsouthwestern.edu' <Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu>>
Subject: {EXTERNAL} [Histonet] stored in 80% isopropanol


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Hi,
If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol  can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol?
Thanks,
Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston, TX 77030

832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org<mailto:BMolinari at texasheart.org>
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