[Histonet] Histonet Digest, Vol 194, Issue 15

Betsy Molinari BMolinari at texasheart.org
Tue Jan 21 05:35:33 CST 2020

Thank you for the reply Amos. I totally agree but the researcher did not inform me until after the deparaffinization was finished. She wanted to hold off on the staining but it was too late and I went ahead with the staining. I was just curious if there was, in fact a procedure to preserve a deparaffinized slide.  

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812 
Email: BMolinari at texasheart.org
texasheart.org <www.texasheart.org> | facebook <www.facebook.com/Texas.Heart.Institute> | twitter <twitter.com/Texas_Heart>
-----Original Message-----
From: Amos Brooks via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Monday, January 20, 2020 5:59 PM
To: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15

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Hi Betsy,
     I wouldn't do it. It's an unnecessary risk to let them dry out. Better to leave it in distilled water if you absolutely must. Ideal to not deparaffinize in the first place if you can't finish the stain. Dried out sections is just a bad plan.

Amos Brooks

On Mon, Jan 20, 2020, 1:00 PM <h-

> Message: 1
> Date: Mon, 20 Jan 2020 12:56:20 +0000
> From: Betsy Molinari <BMolinari at texasheart.org>
> To: "'Histonet at lists.utsouthwestern.edu'"
>         <Histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Deparaffinized slides
> Message-ID:
>         <
> SN6PR10MB28955C0F9DCD3FE5C5FDF3FDCE320 at SN6PR10MB2895.namprd10.prod.out
> look.com
> >
> Content-Type: text/plain; charset="us-ascii"
> Good day and Happy Martin Luther King Day. What are your thoughts on 
> letting deparaffinized slides dry to be used for future use , or keep 
> in DI water for a time? I deparaffinized  some slides this morning, 
> then the researcher called and asked me to hold off on staining them. 
> I am going to continue staining them after explaining the situation to 
> her. But I am just curious if they can be held after being deparaffinized.
> Thank you.
> Betsy Molinari HT(ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> Houston, Texas
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