[Histonet] Pap stains

[S hay]

Hi John but what about ALL of the other solutions in a staining row that
could be potentially contaminated. Why are folks only filtering the dyes?

On Thu, Jan 2, 2020, 12:17 AM John Kiernan <jkiernan at uwo.ca> wrote:

> As a student in the 1960s I was told by my elders and betters to filter
> all staining solutions before using. It was very good advice. Filtration
> does not prolong the life of a stain, but it does remove *crud*, a
> material that can be composed of polymerized dyes and bits of previously
> stained cells or tissues.
>
> Fifty years on, I can identify a few staining solutions that never
> deteriorate into insoluble materials that fall out of solution even after
> 10+ years. Even for these, filtration before use is good laboratory
> practice. Not filtering any stain may therefore be bad laboratory practice.
>
> Happy New Year, * John Kiernan*
> = = =
> ------------------------------
> *From:* Haas, Elizabeth via Histonet <histonet at lists.utsouthwestern.edu>
> *Sent:* 31 December 2019 11:46
> *To:* S hay <susanhay59 at gmail.com>
> *Cc:* histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu
> >
> *Subject:* Re: [Histonet] Pap stains
>
>
> I believe filtering stains daily is a CAP requirement
>
>
> Sent from my iPhone
>
> > On Dec 31, 2019, at 9:23 AM, S hay via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
> >
> > 1. Does everyone filter their pap stains daily?
> > 2. Are you chaining all other reagents daily?
> >
> > Thanks in advance.
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