[Histonet] Pap stains

John Kiernan jkiernan at uwo.ca
Thu Jan 2 00:17:32 CST 2020

As a student in the 1960s I was told by my elders and betters to filter all staining solutions before using. It was very good advice. Filtration does not prolong the life of a stain, but it does remove crud, a material that can be composed of polymerized dyes and bits of previously stained cells or tissues.

Fifty years on, I can identify a few staining solutions that never deteriorate into insoluble materials that fall out of solution even after 10+ years. Even for these, filtration before use is good laboratory practice. Not filtering any stain may therefore be bad laboratory practice.

Happy New Year,  John Kiernan
= = =
From: Haas, Elizabeth via Histonet <histonet at lists.utsouthwestern.edu>
Sent: 31 December 2019 11:46
To: S hay <susanhay59 at gmail.com>
Cc: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Pap stains

I believe filtering stains daily is a CAP requirement

Sent from my iPhone

> On Dec 31, 2019, at 9:23 AM, S hay via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 1. Does everyone filter their pap stains daily?
> 2. Are you chaining all other reagents daily?
> Thanks in advance.
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