[Histonet] Blank Spots on IHC- Bubbles on Bond

[Morken, Timothy]

Greg, thanks!

I have a leica engineer coming in to day to look at it, specifically the fluidics system, so hopefully he’ll see what you mention and fix it.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

From: Greg Dobbin <greg.dobbin at gmail.com>
Sent: Thursday, August 13, 2020 10:20 AM
To: Morken, Timothy <Timothy.Morken at ucsf.edu>; histonet at lists.utsouthwestern.edu
Subject: RE: Blank Spots on IHC- Bubbles on Bond

Hi Tim,
The usual source of bubbles is a deteriorating syringe or lines or valve. Have you observed the syringe while running the Clean Fluidics maintenance function? There can be some "micro" bubbles in the syringe on first draw of a reagent however, if working properly these tiny bubbles quickly disappear in subsequent draws of that reagent. If bubbles persist in the barrel of the syringe throughout the flushing, then you have found the source of the problem. If the bubbles occur with every reagent then the syringe is the problem but if you only see bubbles in one of the reagents then it is a defective valve or line for that reagent.
I hope this was helpful.
Greg

--
Greg Dobbin
1205 Pleasant Grove Rd
RR#2 York,
PE      C0A 1P0

Everything in moderation...even moderation itself!