[Histonet] Blank Spots on IHC- Bubbles on Bond

[Greg Dobbin]

Hi Tim,
The usual source of bubbles is a deteriorating syringe or lines or valve.
Have you observed the syringe while running the Clean Fluidics maintenance
function? There can be some "micro" bubbles in the syringe on first draw of
a reagent however, if working properly these tiny bubbles quickly disappear
in subsequent draws of that reagent. If bubbles persist in the barrel of
the syringe throughout the flushing, then you have found the source of the
problem. If the bubbles occur with every reagent then the syringe is the
problem but if you only see bubbles in one of the reagents then it is a
defective valve or line for that reagent.
I hope this was helpful.
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE      C0A 1P0


*Everything in moderation...even moderation itself**!*