[Histonet] too much formalin time (Curt Tague)
carl.hobbs at kcl.ac.uk
Sun Apr 5 13:39:25 CDT 2020
I trust you and yours are well...
Imho.....it will still be an excellent control, provided that you are processing it to Paraffin wax and use HIER ( antigen retrieval) before IHC/IF.
Sure, as long as it has been optimally fixed ( immersion fixation with no agitation -BAD- leads to zonal variations of positivity)
I have posted images on Histonet site of human tonsil that has remained in fix for a similar time before processing.
Sure, I cannot guarantee all proteins will be retrievable but, in my XP, good fixation is the key....not the length of fixation.
Carl Hobbs FIBMS
Histology and Imaging Manager
Guys Campus, London Bridge
Kings College London
020 7848 6813
More information about the Histonet