[Histonet] too much formalin time (Curt Tague)

Hobbs, Carl carl.hobbs at kcl.ac.uk
Sun Apr 5 13:39:25 CDT 2020

Hi Curt
I trust you and yours are well...
Imho.....it will still be an excellent control, provided that you are processing  it to Paraffin wax and use  HIER ( antigen retrieval) before IHC/IF.
Sure, as long as it has been optimally fixed ( immersion fixation with no agitation -BAD- leads to zonal variations of positivity)
I have posted images on Histonet site of human tonsil that has remained in fix for a similar time before processing.
Sure, I cannot guarantee all proteins will be retrievable but, in my XP, good fixation is the key....not the length of fixation.

Best wishes


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London

020 7848 6813

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