[Histonet] Metals

[Tony Henwood (SCHN)]

Two good screening stains are Mallory and Parker’s Fresh Hematoxylin Stain for Metals and  Timm’s Silver Sulphide Method for Metals. Malloy's results:
Aluminium	Blue-black
Copper	Greenish-blue
Iron	Blue-black
Lead	Blue
Zinc	Blue

For more specific staining:
Aluminon Stain for Aluminium Hydroxide
Walton’s Stain for Aluminium  (Phloxine binds the aluminium)
Bedrick et al (1986) method for Zinc 
Rubeanic Acid Technique for Copper
Rhodanine Technique for Copper 

These methods are quite sensitive but  there are some specificity issues. I can provide further details and references if required. Here are some:

Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59.

Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290.

Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

________________________________________
From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Monday, 30 September 2019 20:49
To: 'histonet at lists.utsouthwestern.edu'
Subject: [Histonet] Metals

Morning all!

I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc.

Thanks much!

Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP)
Centers for Diseases Control and Prevention
1600 Clifton Road NE
MS H18-SB
Bldg. 18, Rm SB-114
Atlanta, GA 30329
404-639-3590

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