[Histonet] Permanent mountant for Oil Red O

[B kB]

Hi Christopher,

You van try Imsol-mount
Vendors are for example Sigmaaldrich or VWR .

After a staining, airdry the solide. Cover with mislopen-mount and let this
dry for a while ( no problem with higher temps). After that, You can cover
it with glass or tape.

Goed luck,
Bert klein brink
Gelre Hospital, Apeldoorn, Netherlands



Op ma 16 sep. 2019 om 06:04 schreef Hagon, Christopher (Health) via
Histonet <histonet at lists.utsouthwestern.edu>

> UNCLASSIFIED
>
> Hello Histonetters,
>
> We do Oil Red O stains on frozen section post mortem tissue, and need to
> find a permanent aqueous mounting medium. We used to use the Thermo-Fisher
> Perm-mount, but can't seem to get it anymore. As they are looking for fat
> deposits, we can't use any solvent based solutions, and the only aqueous
> ones we've found aren't permanent. We have to send the slides off site
> after staining, so the chances of the coverslip moving in transport is
> fairly high.
>
> Does anyone else have this issue and what did you end up using?
>
> Thanks in advance,
>
> Chris Hagon | Senior Scientist, Anatomical Pathology
> ACT Pathology | health.act.gov.au<https://health.act.gov.au/>
> Phone (02) 5124 2874
>
>
>
>
>
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