[Histonet] Detecting copper in H&E staining?

[MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST)]

Perhaps they did an orcein stain with H&E counterstain?  That would make the copper associated protein a dark red-brown colour but with other components looking like an H&E.

Penny Marr
Senior BMS

C/- Histology
Conquest Hospital
St Leonards-on-Sea TN37 7RD

penny.marr at nhs.net

(01424) 758023


-----Original Message-----
From: Jennifer Phinney [mailto:jhill at vet.k-state.edu]
Sent: 10 September 2019 15:57
To: HistoNet
Subject: [Histonet] Detecting copper in H&E staining?

Hello all,
I am in a veterinary histology lab. One of my Pathologists mentioned that at two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) copper deposits stained in the routine H&E slides. Is anyone aware of how this could have been achieved? I assume it would either have to be an additive in the eosin or perhaps something naturally occurring in the tap water rinses?


Thanks for any assistance,
Jennifer Phinney QIHC
Histology Laboratory Administrator
KSVDL



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