[Histonet] Detecting copper in H&E staining?

[Brett Connolly]

I believe it is a rhoda’N’ine stain not rhoda’M’ine. I did it years ago on Alzheimer’s brain sections – works great.

http://www.ihcworld.com/_protocols/special_stains/rhodanine_ellis.htm

Brett Connolly, Phd, HTL(ASCP) happily retired

Sent from Mail for Windows 10

From: Rene J Buesa via Histonet
Sent: Tuesday, September 10, 2019 11:32 AM
To: HistoNet; Jennifer Phinney
Subject: Re: [Histonet] Detecting copper in H&E staining?

 Unfortunately your pathologist is wrong. You will need the "rhodamine" special IHC method to identify copper in tissue sections.René
    On Tuesday, September 10, 2019, 11:08:09 AM EDT, Jennifer Phinney via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Hello all,
I am in a veterinary histology lab. One of my Pathologists mentioned that at two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) copper deposits stained in the routine H&E slides. Is anyone aware of how this could have been achieved? I assume it would either have to be an additive in the eosin or perhaps something naturally occurring in the tap water rinses?


Thanks for any assistance,
Jennifer Phinney QIHC
Histology Laboratory Administrator
KSVDL
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