[Histonet] Mallory-Azan stain

[Manfre, Philip]

The Bielschowsky Silver Stain is a good stain for Purkinje cells, so perhaps fibers as well.
Phil.

Philip Manfre, BA, HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP81-406
770 Sumneytown Pike
West Point, PA 19486
215-652-9750
philip_manfre at merck.com




-----Original Message-----
From: Bob Richmond via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Monday, September 9, 2019 1:24 PM
To: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Mallory-Azan stain

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Betsy Molinari HT, ASCP at the Texas Heart Institute in Houston asks:

>>I have a researcher that wants to stain Purkinje fibers and has 
>>requested
a Mallory-Azan stain. I have no experience with this stain. I have looked online for information but am reaching out to you for personal advice.<<

Well, I'm 80 years old and have never seen a Mallory-Heidenhain azan stain
- it was something you read about in old books when I was young. I have a fair collection of old books, and found a method in Ann Preece's venerable _A Manual for Histologic Technicians_, 3rd ed, 1972. I can scan it for you if nobody else sends you one.

The stain requires aniline ("aniline oil"), which I don't think you're allowed to handle any more - it's a significant hazmat.

Your researcher is probably using a very old reference, and needs to be brought into the 21st century.

Bob Richmond
Samurai Pathologist
Maryville TN
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