[Histonet] Tissue scrolling

[Margaryan, Naira]

I do the same way! Good luck!
Naira

________________________________
From: Colleen Forster <cforster at umn.edu>
Sent: Sunday, September 8, 2019 12:43 PM
To: John Garratt <john.garratt at ciqc.ca>
Cc: Erin McCarthy <erin.mccarthy at tempus.com>; histonet-request <Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Tissue scrolling

We cut alot if these. This is the protocol:

1. The microtome, blade and water bath are cleaned well. We follow
withRNAse Away wipe down as an added precaution.

2. We use a coplin jar of 100% ethanol for forceps, pick or whatever other
tool you might  use that comes into contact with and dip and clean in
between each sample. I actually have 2 sets and put them into the alcohol
and switch out each time.

3 I use the RNAse free tubes to put the curls into.

4. You must wear gloves.

5. If they don't pick the samples up right away they are placed in a -20
freezer for storage.

They have good results.

Respectfully,

Colleen Forster HT(ASCP)
U of MN


On Sun, Sep 8, 2019, 10:36 AM John Garratt via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> The best I can offer is to advise you to wipe the knife holder area down
> with a cleaning solvent and use a fresh blade. Clean forceps with a solvent
> and place the scroll (50 microns) directly into a labelled 1.5mL plastic
> vial. I would be interested on other thoughts on this.
>
> John
>
>
>
> www.ciqc.ca<http://www.ciqc.ca>
>
>
>
>
> ‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
> On Friday, September 6, 2019 3:05 PM, Erin McCarthy via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> > Hello Histonetters,
> >
> > I am curious if any of you perform scrolling of paraffin embedded tissue
> > for molecular studies? If so, what precautions do you take to minimize
> > contamination? I am asking specifically about at the cutting station as
> > that is my area of expertise. But we are looking to pilot this for
> samples
> > that in the past have had large quantities of recuts.
> >
> > Thank you for any information you can provide!
> >
> >
> ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
> >
> > Erin McCarthy, HT (ASCP)
> > Histology Supervisor
> >
> > Tempus Labs
> > 600 W. Chicago Ave.
> > Chicago IL 60654
> > Cell: (708)269-8610
> >
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