[Histonet] Tissue scrolling

[John Garratt]

The best I can offer is to advise you to wipe the knife holder area down with a cleaning solvent and use a fresh blade. Clean forceps with a solvent and place the scroll (50 microns) directly into a labelled 1.5mL plastic vial. I would be interested on other thoughts on this.

John



www.ciqc.ca




‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Friday, September 6, 2019 3:05 PM, Erin McCarthy via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Hello Histonetters,
>
> I am curious if any of you perform scrolling of paraffin embedded tissue
> for molecular studies? If so, what precautions do you take to minimize
> contamination? I am asking specifically about at the cutting station as
> that is my area of expertise. But we are looking to pilot this for samples
> that in the past have had large quantities of recuts.
>
> Thank you for any information you can provide!
>
> ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>
> Erin McCarthy, HT (ASCP)
> Histology Supervisor
>
> Tempus Labs
> 600 W. Chicago Ave.
> Chicago IL 60654
> Cell: (708)269-8610
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