[Histonet] waterbath/paraffin

[Tony Henwood (SCHN)]

Are the tissues taken directly from the molten wax and placed in the wax-containing mold or are they allowed to cool before embedding?

One cause of tissue separation is the difference in temperature between tissue and embedding wax. 
Try taking directly from molten wax and embedding directly in the wax-filled mold.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

________________________________________
From: Betsy Molinari via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Saturday, 26 October 2019 00:49
To: 'Histonet at lists.utsouthwestern.edu'
Subject: [Histonet] waterbath/paraffin

Hi,
When I place my sections on the waterbath the paraffin pulls away from it. Leaving just the edge of the tissue. It is very weird. I have tried at different temps . The tissue itself is fine so I do not believe it is the processing. I use the same paraffin for processing as well as embedding. I use Fisher Histoplast IM.  I was considering melting a block or two down and embedding them in another paraffin from another lab.
Suggestions?

Betsy Molinari HT, ASCP
Texas Heart Institute
6770 Bertner Ave.
O-511
Houston, TX 77030
832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
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