[Histonet] waterbath/paraffin

[Betsy Molinari]

Hi,
When I place my sections on the waterbath the paraffin pulls away from it. Leaving just the edge of the tissue. It is very weird. I have tried at different temps . The tissue itself is fine so I do not believe it is the processing. I use the same paraffin for processing as well as embedding. I use Fisher Histoplast IM.  I was considering melting a block or two down and embedding them in another paraffin from another lab.
Suggestions?

Betsy Molinari HT, ASCP
Texas Heart Institute
6770 Bertner Ave.
O-511
Houston, TX 77030
832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: BMolinari at texasheart.org
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