[Histonet] Artifact IHC

[Bliven, Laura M]

I have seen an artifact on an Estrogen IHC slide, although rare, our pathologists have also seen it on outreach slides.
It appears somewhat granular and/or dot-like and it is not on the Progesterone slide.
The Estrogen uses a high pH and our Progesterone does not. I assume it's caused by the high pH epitope retrieval, but would like a reference to this theory.
Any ideas?

Laura
Bliven.laura at marshfieldclinic.org

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