[Histonet] HSV-2 antibody - Cell Marque was not the problem

[Sheeder, Christopher]

Beth,
You have honor and integrity. Something seldom seen these days.

Chris Sheeder
Seattle Children's Hospital

-----Original Message-----
From: O'Neil, Beth <beth.oneil1 at wvumedicine.org>
Sent: Wednesday, July 03, 2019 11:20 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem

Last week I posted an inquiry about having problems with Cell Marque's HSV-2 polyclonal antibody.  I have to retract my statement that it was Cell Marque using a different antibody source.   After spending two weeks troubleshooting my stainer, troubleshooting the positive control slides, yelling at my Cell Marque rep, etc.  I found out that it was the positive control slide and not the antibody.  When I originally suspected the positive QC slides (from Cancer Diagnostics), I requested a different lot number.  This new lot also failed to show positive staining.  Long story short, another call to Cancer Diagnostics finally resulted in receiving confirmation that they are having problems with their HSV-2 control slides.  It was my misfortune to have opened a new box of QC slides at the same time as opening a new vial of HSV-2 antibody which resulted in two weeks of headaches.  I will say that Cell Marque/Millipore was very supportive through this.  So, for those of you who are using Cancer Diagnostics HSV-2 control slides, they are working on a resolution and will replace their "bad" slides.  I was told that the staining is "hit or miss, "  hopefully you all have the "hit" slides.

Beth Oneil, WVU Medicine




 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.