[Histonet] guinea pig IHC

[Patsy Ruegg]

In my experience it is not that GP have a higher peroxidase level, it is frozen sections in general that cannot be blocked with h202, unless they are fixed for a long time in formalin.  What are others experiences with h202 blocking on frozen sections.  I always used an IHC detection system that did not require h202 blocking for frozen sections.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm at hotmail.com


________________________________
From: Jan Shivers <shive003 at umn.edu>
Sent: Tuesday, January 29, 2019 12:58 PM
To: histonet
Subject: [Histonet] guinea pig IHC

Has anyone ever performed IHC on frozen sections of guinea pig tissue?  I
am experiencing an enormous amount of bubbling when doing the peroxidase
blocking step, even though I'm only using a 0.3% concentration of H2O2.
And when I say 'enormous', I mean it's like continuous champagne bubbles
rising out of the tissue, even after 20 minutes in the H2O2 solution.

I can't find anything in the literature that mentions guinea pigs having a
higher peroxidase content in their tissues.

Thanks for any help that anyone can provide.

Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 at umn.edu

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