[Histonet] guinea pig IHC
cforster at umn.edu
Tue Jan 29 14:12:59 CST 2019
I haven't done frozen stains on GP only FFPE...interested in what others
have to say.
On Tue, Jan 29, 2019 at 1:58 PM Jan Shivers via Histonet <
histonet at lists.utsouthwestern.edu> wrote:
> Has anyone ever performed IHC on frozen sections of guinea pig tissue? I
> am experiencing an enormous amount of bubbling when doing the peroxidase
> blocking step, even though I'm only using a 0.3% concentration of H2O2.
> And when I say 'enormous', I mean it's like continuous champagne bubbles
> rising out of the tissue, even after 20 minutes in the H2O2 solution.
> I can't find anything in the literature that mentions guinea pigs having a
> higher peroxidase content in their tissues.
> Thanks for any help that anyone can provide.
> Jan Shivers
> Senior Scientist
> IHC/Histology Section Manager
> Pathology Teaching Program
> Veterinary Diagnostic Laboratory
> University of Minnesota
> 1333 Gortner Ave.
> St. Paul, MN 55108
> shive003 at umn.edu
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Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB 612-626-1930
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