[Histonet] guinea pig IHC

Colleen Forster cforster at umn.edu
Tue Jan 29 14:12:59 CST 2019


I haven't done frozen stains on GP only FFPE...interested in what others
have to say.


On Tue, Jan 29, 2019 at 1:58 PM Jan Shivers via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Has anyone ever performed IHC on frozen sections of guinea pig tissue?  I
> am experiencing an enormous amount of bubbling when doing the peroxidase
> blocking step, even though I'm only using a 0.3% concentration of H2O2.
> And when I say 'enormous', I mean it's like continuous champagne bubbles
> rising out of the tissue, even after 20 minutes in the H2O2 solution.
> I can't find anything in the literature that mentions guinea pigs having a
> higher peroxidase content in their tissues.
> Thanks for any help that anyone can provide.
> Jan Shivers
> Senior Scientist
> IHC/Histology Section Manager
> Pathology Teaching Program
> Veterinary Diagnostic Laboratory
> University of Minnesota
> 1333 Gortner Ave.
> St. Paul, MN  55108
> 612-624-7297
> shive003 at umn.edu
> *Confidentiality Notice: This message, together with any attachments, is
> intended only for the use of the individual or entity to which it is
> addressed and may contain confidential or privileged information. If you
> think you have received this message in error, please advise the sender and
> then delete this message and any attachments immediately.*
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB      612-626-1930

*If submitting histology request please also forward to Lori Holm at
holml at umn.edu <holml at umn.edu>*

More information about the Histonet mailing list