[Histonet] Opinion on dye on biopsies

Tony Henwood (SCHN) tony.henwood at health.nsw.gov.au
Wed Jan 9 17:09:29 CST 2019

One article that might be useful is:

Surprenant D, Garib G, Hutchens K, Dreifke M, Speiser J, Winterfield L, Peterson A, Krol C, Adams W, Tung R. Novel Use of Preoperative Epidermal Coloring of Very Small Dermatological Specimens-Protocol for Reduction of Lost Specimens. The American Journal of Dermatopathology. 2016 Jul 1;38(7):510-2.

I would expect that this would colour the squamous cell layer of the oesophageal mucosa.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: Gareth Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, 10 January 2019 9:15 AM
To: Histonet at lists.utsouthwestern.edu
Subject: [Histonet] Opinion on dye on biopsies

So, I work in a small GI lab, and I put Eosin in my first formalin on my processor.  My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting.  But, unfortunately, the esophagus tissues do not absorb the eosin much.  Anyway, the hospital lab I work, part-time, in has started using hematoxylin to help see their biopsies.  I happen to embed there and I think it just makes a big mess and the tissue does not absorb much of the stain.
What are other labs doing to aid in making their small biopsies easier to see?  What are pros and cons to doing this, in your opinion?

*Ms. Gareth B. Davis*, B.S., HT, QIHC  (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364
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