[Histonet] Multi-Tumor Controls for IHC Validation

John Garratt john.garratt at ciqc.ca
Tue Jan 1 16:13:17 CST 2019

For a basic multi-tissue control I recommend tonsil, liver, appendix and pancreas. Ensure you standardize fixation and processing time for the tissues you use

I suggest that labs use iCAPCs (immunohistochemistry critical assay performance controls) for developing your controls. This article (Standardization of Positive Controls in Immunohistochemistry) and will explain iCAPCs.

All the best for 2019



‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Monday, December 31, 2018 10:39 AM, Miranda Giorgi via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Hello,
> Does anyone use multi tumor tissue controls for their IHC validations? If so, what do you use in them?
> We have been staining multi-tumor controls with our validation sets on top of the 10 positive and 10 negative cases to test specificity, but we are wondering if this is necessary. Plus, the blocks are sometimes difficult to maintain. I'd love to hear what other labs are doing.
> Thank you,
> Miranda Giorgi, HTL (ASCP)cm
> Histology Manager
> Incyte Diagnostics
> 509-892-2744
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