[Histonet] Muscle Enzyme Histochemistry

[Bryan Llewellyn]

Hi,
I presume you are talking about light microscopy. Glutaraldehyde is 
often used for electron microscopy. If that is the case, ignore this.

Glutataraldehyde fixes similarly to formalin, so the morphology should 
not be much different. However, it leaves the tissue with free aldehyde 
groups and these can react with Schiff's reagent or silver reductions. 
That means if doing a PAS or methenamine silver you should first (before 
oxidation) do an aldehyde block. I recommend aniline-acetic acid for 30 
minutes, although others work as well. If you don't you will have a dark 
background. It is unlikely that enzyme histochemistry will be 
successful, but you never know. Esterase may still be demonstrable, 
possibly, but the ATPase iso-enzymes are unlikely to survive.

Go to http://stainsfile.info and read the pages on glutaraldehyde for 
more information about it.

Bryan Llewellyn


MONICA D. LOCKHART via Histonet wrote:
> Hello Histology World!!!
>
> Can muscle placed in Gluteraldehyde be processed for structure?  In this situation, all tissue was placed in Glut and we need to perform Enzyme Histochemistry but we have no fresh tissue.
>
> Thanks for your help.
>
> Monica D. Lockhart, BBA, HT (ASCP) PBT
> Supervisor Clinical Labs Histology
> Loyola University Medical Center
> 2160 S. First Ave, Bldg 110 Rm 2290
> Maywood, IL  60153
> (o) 708.327.2608
> (c) 708.692.8361
> monica.lockhart at luhs.org
>
>
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