[Histonet] Air pocket in paraffin blocks

[Theresa Dalton]

Oh no!   Absolutely re-embed.  

Sent from my iPhone

> On Feb 22, 2019, at 4:57 AM, Victoria Baker via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Yes, it does. The air bubble went directly under the tissue.
> 
> Vikki
> 
> On Thu, Feb 21, 2019, 8:43 PM Jennifer Phinney <jhill at vet.k-state.edu>
> wrote:
> 
>> Vikki,
>> Did the air bubble affect the section quality on the slide? If not, would
>> it have been worth the delay to the case in order to melt and re-embed the
>> tissue?
>> 
>> I only re-embed blocks with air bubbles if it will affect the quality of
>> the slide myself.
>> 
>> 
>> Jennifer
>> 
>> -----Original Message-----
>> From: Victoria Baker via Histonet <histonet at lists.utsouthwestern.edu>
>> Sent: Thursday, February 21, 2019 4:49 PM
>> To: histonet at lists.utsouthwestern.edu
>> Subject: [Histonet] Air pocket in paraffin blocks
>> 
>> Hi,
>> 
>> When I was trained to do embedding there were many things that the
>> professor stressed to me need to be done in order to have the tissue block
>> acceptable for sectioning.  One of these was air bubbles.
>> 
>> Recently we had a new tech embed a derm block that had an bubble that was
>> pretty big.  The other (experienced) techs didn't think anything of it
>> either and sectioned it.  When I got the block for IHC screening I made a
>> QA form stating that the block should have been re-embedded before giving
>> it to be sectioned, or when the first tech sectioned it could have repaired
>> the block or melted it down.  This air bubble was big enough to be seen so
>> I don't think it could have been missed - unless the block wasn't checked
>> right after embedding.
>> 
>> What has me a little upset is that no one seemed to care about this.
>> 
>> I would really appreciate some feedback about this from other people.
>> 
>> Thanks in advance.
>> 
>> Vikki
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