[Histonet] Air pocket in paraffin blocks

[Victoria Baker]

Yes, it does. The air bubble went directly under the tissue.

Vikki

On Thu, Feb 21, 2019, 8:43 PM Jennifer Phinney <jhill at vet.k-state.edu>
wrote:

> Vikki,
> Did the air bubble affect the section quality on the slide? If not, would
> it have been worth the delay to the case in order to melt and re-embed the
> tissue?
>
> I only re-embed blocks with air bubbles if it will affect the quality of
> the slide myself.
>
>
> Jennifer
>
> -----Original Message-----
> From: Victoria Baker via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Thursday, February 21, 2019 4:49 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Air pocket in paraffin blocks
>
> Hi,
>
> When I was trained to do embedding there were many things that the
> professor stressed to me need to be done in order to have the tissue block
> acceptable for sectioning.  One of these was air bubbles.
>
> Recently we had a new tech embed a derm block that had an bubble that was
> pretty big.  The other (experienced) techs didn't think anything of it
> either and sectioned it.  When I got the block for IHC screening I made a
> QA form stating that the block should have been re-embedded before giving
> it to be sectioned, or when the first tech sectioned it could have repaired
> the block or melted it down.  This air bubble was big enough to be seen so
> I don't think it could have been missed - unless the block wasn't checked
> right after embedding.
>
> What has me a little upset is that no one seemed to care about this.
>
> I would really appreciate some feedback about this from other people.
>
> Thanks in advance.
>
> Vikki
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