[Histonet] ER/PR question

[Cartun, Richard]

If the tumor is ER/PR negative the first thing I do is to look for an internal positive control (immunoreactive benign breast epithelium).  In my experience, the majority of these cases have internal positive controls to validate the negative ER/PR results.  When I don't see internal positive controls, and the HER2 is negative, I recommend that the testing be repeated on excisional tumor for confirmation.  This is why it's important to submit adjacent benign breast tissue along with the tumor tissue when grossing.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
Richard.cartun at hhchealth.org

-----Original Message-----
From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, February 08, 2019 8:52 AM
To: karen.heckford at dignityhealth.org; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] ER/PR question

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Hi Karen,

As mentioned by others "decay" is not likely going to be an issue. More

concerning for you could be not knowing how those tissues were handled

prior to processing 10 years ago.



Presumably, you now track cold ischemic times and have standardized your

fixation protocols for breast tissues and you have validated your IHC

procedures with these current parameters. If your lab was like many others

10 years ago, these parameters were probably not maintained so rigorously.

So interpretation of a negative result may be problematic.



Having said that, there is the internal control for both ER and PR. It

seems to me that if the internal control is staining adequately, then

perhaps interpretation will not be an issue. I am curious, does anyone have

a different take on relying the internal control as a guide in this

particular situation??

Greg



--

*Greg Dobbin*

1205 Pleasant Grove Rd

RR#2 York,

PE      C0A 1P0





*Everything in moderation...even moderation itself**!*

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