[Histonet] plural fluid prep

[John Garratt]

The cells have been living quite nicely in a natural culture medium at 37c so putting the pleural fluid at 4c will preserve the cells for a number of days. I do not think I would want to go past 3 to 4 days, but that is just the limit of my experience revisiting old ascitic fluid specimens.
You could use alcohol to preserve the specimen longer but not knowing what your experiment is I am loath to offer an opinion on that.

John

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On Wed, Dec 18, 2019 at 9:51 AM, Michelle Jamison via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Hi All,
> I work in a research lab where we are using plural fluid to proof a
> method. Recently we have received some, and are unsure how long it will
> be ok in the fridge, or if we should add some preservation fluid to it.
> Can anyone advise please.
> Thank you in advance, and Happy Holidays to all!!
> --
>
> Best of all things to you,
>
> Michelle Jamison
>
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> Tel : +1.435.752.6011 Ext. 435.227.1474
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> m.jamison at elitechgroup.com <mailto:> • www.elitechgroup.com
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