[Histonet] Delay in embedding?

[Garrey Faller]

I’ve done it a handful of times when my only Histotech called in sick.
No effect on quality.

Garrey

Sent from my iPhone

> On Dec 18, 2019, at 2:24 AM, Jennifer Saunders via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> I don’t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time. Jen Saunders 
> 
> 
> Sent from Yahoo Mail for iPhone
> 
> 
> On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Hi Paula,
> 
> We routinely do this, especially for our fetal autopsy blocks.
> We are then able to process and let them set at room temp until we are able to embed and cut.
> Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities.
> It is more efficient for us.
> 
> Morphology, staining and immunohistochemistry is not affected.
> 
> It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected.
> 
> Take home point - do not overcook your tissues.
> 
> 
> 
> Regards 
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
> Principal Scientist, the Children’s Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney 
> Tel: 612 9845 3306 
> Fax: 612 9845 3318 
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 
> 
> 
> -----Original Message-----
> From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
> Sent: Wednesday, 18 December 2019 7:34 AM
> To: P Sicurello <patpxs at gmail.com>
> Cc: HistoNet <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Delay in embedding?
> 
> We have done this - only on an emergency basis. 
> 
> Sent from my iPhone
> 
>> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>> 
>> Good Morning Listers,
>> 
>> 
>> 
>> How many out there will process tissue and then leave the cassettes at 
>> room temperature and embed it at a later time (hours or the next day)?
>> 
>> 
>> 
>> Please send me your opinions about doing this.  I think it’s a bad 
>> idea, others I speak with disagree.
>> 
>> Sincerely,
>> 
>> Paula Sicurello, HTL (ASCP)CM
>> 
>> Histotechnology Specialist
>> 
>> UC San Diego Health
>> 
>> 9300 Campus Point Drive
>> 
>> La Jolla, CA 92037
>> (P): 858-249-5610
>> 
>> 
>> 
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