[Histonet] Delay in embedding?

[Jennifer Saunders]

I don’t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time. Jen Saunders 


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On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet <histonet at lists.utsouthwestern.edu> wrote:

Hi Paula,

We routinely do this, especially for our fetal autopsy blocks.
We are then able to process and let them set at room temp until we are able to embed and cut.
Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities.
It is more efficient for us.

Morphology, staining and immunohistochemistry is not affected.

It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected.

Take home point - do not overcook your tissues.



Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, 18 December 2019 7:34 AM
To: P Sicurello <patpxs at gmail.com>
Cc: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Delay in embedding?

We have done this - only on an emergency basis. 

Sent from my iPhone

> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Good Morning Listers,
> 
> 
> 
> How many out there will process tissue and then leave the cassettes at 
> room temperature and embed it at a later time (hours or the next day)?
> 
> 
> 
> Please send me your opinions about doing this.  I think it’s a bad 
> idea, others I speak with disagree.
> 
> Sincerely,
> 
> Paula Sicurello, HTL (ASCP)CM
> 
> Histotechnology Specialist
> 
> UC San Diego Health
> 
> 9300 Campus Point Drive
> 
> La Jolla, CA 92037
> (P): 858-249-5610
> 
> 
> 
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