[Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC?

[Brett Connolly]

Merissa,
I have done tons of IHC assays on frozen tissues, including many procured from commercial tissue banks. My experience has been that the most of the time the microscopic morphology is terrible (abysmal if it is brain tissue). Cells are always blown open from slow freezing. It’s bad enough that there is a 2 week PMI…add to that storage at 4C instead of at least -20C (best would be -80C) and IMO it’s a recipe for failure. Wouldn’t touch that tissue no way, no how…well maybe if they paid ME I would take it and try to use it for autoradiography or something.
Just my opinion.

Brett Connolly HTL (ASCP), PhD

Sent from Mail for Windows 10

From: M.O. via Histonet
Sent: Thursday, August 22, 2019 11:24 AM
To: Histonet
Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC?

Good morning!

We are working with a tissue bank that is only able to procure tissue
blocks from donors that have postmortem intervals of 2wks or more. The
storage conditions are in a cooler (I believe 4C), the block will be
procured and frozen and we will harvest the tissue from that block once we
thaw it. We plan to use these samples for histological purposes only.

Has anyone had success with IHC on donors/tissues that were stored in 4C
for 2wks or more? Will the integrity of the cells and proteins be okay?

Thank you,
Merissa
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