[Histonet] biopsy staining

[Betsy Molinari]

I will try the safranin O on two samples. Also, toluidine blue has been suggested. Do you have any experience with it?


Betsy Molinari
Sr. Histology Research Technician
CV Pathology

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812 
Email: BMolinari at texasheart.org
texasheart.org | facebook | twitter
-----Original Message-----
From: Lynette Pavelich [mailto:lynettepav at gmail.com] 
Sent: Friday, April 26, 2019 9:20 AM
To: Betsy Molinari <BMolinari at texasheart.org>
Subject: Re: [Histonet] biopsy staining

*** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe.
________________________________


I can’t think of a reason why it wouldn’t work or hurt. Give it a try!  Dab off the alcohol and mark. Let us know how it worked out in the end!

> On Apr 26, 2019, at 10:04 AM, Betsy Molinari <BMolinari at texasheart.org> wrote:
>
> That was my concern as well. Since the tissue is already in 70% ETOH can it still be marked?
>
>
> Betsy Molinari
> Sr. Histology Research Technician
> CV Pathology
>
> Texas Heart Institute
> 6770 Bertner Avenue, MC 1-283
> Houston, TX 77030
>
> Office: 832-355-6524 | Fax: 832-355-6812
> Email: BMolinari at texasheart.org
> texasheart.org | facebook | twitter
> -----Original Message-----
> From: Lynette Pavelich [mailto:lynettepav at gmail.com]
> Sent: Friday, April 26, 2019 7:55 AM
> To: Betsy Molinari <BMolinari at texasheart.org>
> Subject: Re: [Histonet] biopsy staining
>
> *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe.
> ________________________________
>
>
> I would not choose eosin then because of its fluorescent properties.
>
> Lynette Pavelich, HT(ASCP), QIHC
>
> Sent from my iPhone
>
>> On Apr 26, 2019, at 8:21 AM, Betsy Molinari via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>>
>> Hi,
>> I have researched the archives but still have concerns. I have some very small pieces of tissue that are in 70% ETOH.  Should I mark them with eosin or safranin O? From what I have read the tissues were still in formalin, and mine are already in alcohol. Can they still be marked? At the moment they are not planning to do florescence, but I would like to keep that option open.
>> Thanks,
>> Betsy Molinari, HT (ASCP)
>> Texas Heart Institute
>> 6770 Bertner Ave
>> Houston, TX 77030
>> 832-355-6524 (lab)
>> 832-355-6812 (fax)
>>
>> Betsy Molinari
>> Sr. Histology Research Technician
>> CV Pathology
>>
>> Texas Heart Institute
>> 6770 Bertner Avenue, MC 1-283
>> Houston, TX 77030
>>
>> Office: 832-355-6524 | Fax: 832-355-6812
>> Email: BMolinari at texasheart.org
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