[Histonet] [EXTERNAL] Retic staining

[Perl , Alison]

Hi Charles
I've had very good luck in the past with Poly Scientific's Gomori's retic kit, which is ready-to-use (no ammoniacal preparation!) and very reliable. I haven't used AMT's. I wonder if anything changed in the transition to StatLab ownership? Making the ammoniacal generally seems to be the trickiest part...

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager 
(914) 302-8424
aperl at cmmedical.com

-----Original Message-----
From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Monday, April 22, 2019 2:57 PM
To: Histo List
Subject: [EXTERNAL] [Histonet] Retic staining

I am having trouble getting my retic stain to come out strong. It is
working but Pathologists are saying they shouldn't have to go to high power
just to see it (and I agree).

I am using a brand new American MasterTech Chandler's Precision kit. I have
followed all the steps correctly (one difficulty is getting the Ammonium
hydroxide to go drop by drop so this may be the issue).

I have tried staining longer in ammoniacal silver. more time in 20%
formalin,  more and less time in gold chloride and sodium thiosulfate.

If anyone has any suggestions or uses this kit with time modifications
please let me know. Willing to try anything to get it working.


-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately.