[Histonet] [Histonet) Ki67 control

[John Garratt]

My preference, whenever possible, is to steer people towards using normal tissues for controls in IHC. They are consistent in quality and appearance and fixation and processing can be better controlled.
I suggest you use Appendix for Ki67 and evaluate the lymphoid follicles plus there are some positive staining cells in the crypts.
Should you feel the need to use tumour and quantify the amount staining you could use Mantle Cell Lymphoma. This should give reasonably accurate consistency of Ki67 count through the block.
See the article "Standardization of Positive Controls.........."   https://www.researchgate.net/publication/282488454_Standardization_of_Positive_Controls_in_Diagnostic_Immunohistochemistry

I am most interested in "selling" the concept of iCAPCs ( immunohistochemistry Critical Assay Performance Controls) and any feedback is appreciated.

John

www.ciqc.ca

‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Monday, April 8, 2019 11:17 AM, Dessoye, Michael via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Hello Histonet!
>
> Looking for some advice regarding ER/PR/Her2 controls. I'm able to buy commercially TMA blocks containing varying levels of positivity for ER, PR, and Her2. This work nicely because you can have a negative and varying levels of positive control as a same-slide control. But our docs would like to score Ki-67 as well. Currently, they are just doing pos/neg for Ki67.
>
> It will take some time to collect cases and create a similar control, so I'm wondering what y'all are using? Is there anything commercially available? Or is everybody just collecting cases with varying degrees of positive?
>
> Thanks,
> Mike
>
> Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.netmailto:mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484
>
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