[Histonet] Elephant Tissues

Fri Sep 7 02:56:00 CDT 2018

Hello Jennifer,

I do not have experiences with elephant tissues, but with other tissues that have thick connective fibres. Sodium hydroxide may help, however, I would recommend resin embedding, too. For this purpose Technovit 7100 would be a good and easy to use resin. Its easy to infiltrate and can be cut with rotary microtomes with some special knifes, and there are many stains that work on Technovit 7100 embedded specimens. Please let me know, if you need further information. We have a local dealer in US.

Kind regards
Michael

> Am 06.09.2018 um 16:41 schrieb Jennifer Phinney via Histonet <histonet at lists.utsouthwestern.edu>:
> 
> Hi Paula,
> One of my pathologists thinks it could be the collagen in the tissues making them difficult to cut.  We have tried nair, fabric softener, and even decaling the tissues and nothing helps.  The tissues shred immediately when trying to section making it impossible in some cases to actually get a slide. When I melted a particularly difficult block down to separate out the different tissues, it turned out to be the lymph node that was the worst.
> 
> We are going to try some of the cream you use to soften scar tissues to see if that has any effect.
> 
> As a veterinary histology lab we routinely work on a variety of different species, and so far the elephant tissue is the only one giving us problems.
> 
> Thanks for everyone’s help,
> Jennifer
> 
> From: P Sicurello <patpxs at gmail.com>
> Sent: Wednesday, September 5, 2018 4:46 PM
> To: Jennifer Phinney <jhill at vet.k-state.edu>
> Cc: HistoNet <Histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Elephant Tissues
> 
> Jennifer,
> 
> I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, giraffe, and even human mummy tissues.  With the exception of the mummy tissue being a bit dry, they all embedded and cut like human tissue.
> 
> What is it that is making them hard to cut?
> 
> Sincerely,
> 
> Paula Sicurello, HTL (ASCP)CM
> 
> Histotechnology Specialist
> 
> UC San Diego Health
> 
> 200 Arbor Drive
> 
> San Diego, CA 92103
> 
> (P): 619-543-2872
> 
> 
> 
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> 
> On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:
> Hello Histonetters,
> Does anyone have experience processing and cutting elephant tissues?  Any tips, tricks, or advice?  My lab has had some elephant cases recently and the tissues are unexpectedly (to us) difficult to cut.
> 
> Thanks for any help,
> Jennifer Phinney QIHC
> Kansas State University
> Veterinary Diagnostic Lab
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