[Histonet] Elephant Tissues

[Histology]

Hi Jennifer,

If it is skin extending the fixing and processing time should cure any processing issues. If after this it’s still not working a short pre fix in khales fixative and surface softening before sectioning with 0.02% sodium hydroxide may help.

Hope this helps!

Stuart Beaver BSc(Hons)
Head of Veterinary Histology/Cytology
+447568543761

On 5 Sep 2018, at 22:46, P Sicurello <patpxs at gmail.com<mailto:patpxs at gmail.com>> wrote:

Jennifer,

I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale,
giraffe, and even human mummy tissues.  With the exception of the mummy
tissue being a bit dry, they all embedded and cut like human tissue.

What is it that is making them hard to cut?

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet <
histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:

Hello Histonetters,
Does anyone have experience processing and cutting elephant tissues?  Any
tips, tricks, or advice?  My lab has had some elephant cases recently and
the tissues are unexpectedly (to us) difficult to cut.

Thanks for any help,
Jennifer Phinney QIHC
Kansas State University
Veterinary Diagnostic Lab
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