[Histonet] Unstained slides

[Ana Maluenda]

Hi all,

It has been very interesting reading all your comments on unstained slides. This has been a forever discussion I always have everywhere I go in different research institutes. So expanding the topic, I wonder what's everyone's opinion on unstained cryosections? How long are they reliable to be used for IHC?

Kind regards,

Ana

Ana Maluenda
Research Assistant
Atherothrombosis and Vascular Biology Laboratory

Baker Heart and Diabetes Institute
75 Commercial Road, Melbourne VIC 3004
P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W www.baker.edu.au


-----Original Message-----
From: Hobbs, Carl [mailto:carl.hobbs at kcl.ac.uk]
Sent: Tuesday, 4 September 2018 4:54 AM
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Unstained slides

I agree: cut only the sections needed.
Saves space.
Sure, you lose several sections of tissue when cutting more sections.
That is acceptable because, if this "oxidation" theory is true, then the initial sections will be no good.
However, careful organisation of exptl procedure before actual cutting will work very well.

Actually, not many Ags get "oxidised"....for eg: I can demonstrate GFAP in sections that are a year old ( sure, they are stored at 4C just in case) These slides are used for Yr 1 BSc practicals and are consistently positive.
Nobody knows why some Ags ( and not others) lose their antigenicity, imho Oxidation is a vague reasoning.
Just like nobody really knows why HIER works: however, I am in the dipole moment school of thought, rather than the Ca++ skool Sure, in Formalin-fixed specimens.

Curious-illy

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6813

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