[Histonet] Unstained slides

[Hobbs, Carl]

I agree: cut only the sections needed.
Saves space.
Sure, you lose several sections of tissue when cutting more sections.
That is acceptable because, if this "oxidation" theory is true, then the initial sections will be no good.
However, careful organisation of exptl procedure before actual cutting will work very well.

Actually, not many Ags get "oxidised"....for eg: I can demonstrate GFAP in sections that are a year old ( sure, they are stored at 4C just in case)
These slides are used for Yr 1 BSc practicals and are consistently positive.
Nobody knows why some Ags ( and not others) lose their antigenicity, imho
Oxidation is a vague reasoning.
Just like nobody really knows why HIER works: however, I am in the dipole moment school of thought, rather than the Ca++ skool
Sure, in Formalin-fixed specimens.

Curious-illy

Carl  
 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813