[Histonet] Repeats in IHC

[Terri Braud]

Our repeat limits differ.  If it is due to tissue falling off, we will try at least 3 times 
If it is due to unexpected, but within control, we will repeat it 2 times
Then if it is still problematic for the above 2, we will send out the block for staining.
If it is due to technical error (wrong block, wrong stain) we will perform the stain until correct, and it better not be more than once, LOL

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Today's Topics:

   1. Histotechs abroad? (Wellen, Terry :LLS Lab)
   2. Re: Frozen sections and cold acetone (Hobbs, Carl)
   3. REPEATS IN IHC (Kurth Virginia L.)
   4. Re: REPEATS IN IHC (Rene J Buesa)


Message: 3
Date: Fri, 26 Oct 2018 20:07:22 +0000
From: Kurth Virginia L. <VKurth at uwhealth.org>
Subject: [Histonet] REPEATS IN IHC

Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too:  The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH.  Thanks Ginny


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Message: 4
Date: Sat, 27 Oct 2018 13:48:06 +0000 (UTC)
From: Rene J Buesa <rjbuesa at yahoo.com>
To: "Kurth Virginia L." <VKurth at uwhealth.org>,
	"histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] REPEATS IN IHC
Message-ID: <1773692175.85882.1540648086619 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

Ginny:It seems you are asking for a "problem" average and that will vary per laboratory and how the whole process is carried out.Section washed out from the slide usually is consequence of poor processing or sectioning and has nothing to do with IHC, although, perhaps, a wrongly performed HIER can cause it.Wrong block is a mistake also having nothing to do with IHC and is always caused by an inattentive HT not paying attention to the process.My point is that, essentially, the lab should try to get to "0" mistakes and any one has to be documented in the QC record but it will be interesting to find out the "mistakes rate" you find and I think you should share it with all.Ren? 

    On Friday, October 26, 2018 4:41 PM, Kurth Virginia L. via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 
 Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too:? The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH.? Thanks Ginny _______________________________________________
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