[Histonet] TMA slides for IHC validation

[Laurie Redmond]

I think they usually include negative tissue in the TMA's.  Check with Biomax on your specific slides.

Laurie Redmond


-----Original Message-----
From: Dessoye, Michael via Histonet <histonet at lists.utsouthwestern.edu>
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Sent: Mon, Oct 15, 2018 10:34 am
Subject: [Histonet] TMA slides for IHC validation

I'm using TMA slides from US Biomax to assist with IHC validation.  I'm working on p120 on a Benchmark Ultra and BR2410 slides from Biomax.  The cases are all invasive lobular carcinoma, which should all stain positively, but only about half are staining positive.  I sent an identical slide to our reference lab and the staining matches my own.

Since it's the first time I've worked with TMA slides, am I missing something obvious?  Are the tissue cores generally prepared in the same fashion?  Any reasons why I'm seeing inconsistent staining of tissue that should all be positive?  All regular cases pulled of invasive lobular carcinoma are staining appropriately, just something unusual with these TMA slides.

Any advice is appreciated!  Thanks!

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net<mailto:mjdessoye at commonwealthhealth.net> | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484

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