[Histonet] Inconsistent H & E Staining

[John Garratt]

A quick question: Are you using any recycled reagents? ie xylene, alcohol

John


www.ciqc.ca

‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
On Wednesday, November 14, 2018 12:24 PM, MONICA D. LOCKHART via Histonet <histonet at lists.utsouthwestern.edu> wrote:

> Hello Histo Fam!!
>
> Please help!
>
> My facility is experiencing frequent H & E staining problems. Everything from cloudy cells on routine GI to "The Blank Look" on PNB's.
>
> We have made changes in some of the obvious areas like cassette whole size and discontinued the use of sponges at grossing. However, with our biopsies, we still see varying results in our H&E staining.
>
> Though this is not an exhaustive list of all the things we have encountered or the number of changes we have made, hopefully this will give you an idea of what we are seeing and maybe you have ran across this problem and can share your wisdom.
>
> Any comments would be appreciated and most welcome.
>
> Monica D. Lockhart, BBA, HT (ASCP) PBT
> Supervisor Clinical Labs Histology
> Loyola University Medical Center
> 2160 S. First Ave, Bldg 110 Rm 2290
> Maywood, IL 60153
> (o) 708.327.2608
> (c) 708.692.8361
> monica.lockhart at luhs.org
>
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