[Histonet] Inconsistent H & E Staining

[Caroline Miller]

Have you checked your dewaxing solutions? I have sometimes seen this when
slides are not fully dewaxed.

yours,
mills
ᐧ

On Wed, Nov 14, 2018 at 12:34 PM MONICA D. LOCKHART via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello Histo Fam!!
>
> Please help!
>
> My facility is experiencing frequent H & E staining problems.  Everything
> from cloudy cells on routine GI to "The Blank Look" on PNB's.
>
> We have made changes in some of the obvious areas like cassette whole size
> and discontinued the use of sponges at grossing.  However, with our
> biopsies, we still see varying results in our H&E staining.
>
> Though this is not an exhaustive list of all the things we have
> encountered or the number of changes we have made, hopefully this will give
> you an idea of what we are seeing and maybe you have ran across this
> problem and can share your wisdom.
>
> Any comments would be appreciated and most welcome.
>
>
> Monica D. Lockhart, BBA, HT (ASCP) PBT
> Supervisor Clinical Labs Histology
> Loyola University Medical Center
> 2160 S. First Ave, Bldg 110 Rm 2290
> Maywood, IL  60153
> (o) 708.327.2608
> (c) 708.692.8361
> monica.lockhart at luhs.org
>
>
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-- 
Caroline Miller (mills)
Director of Biology

www.3Scan.com
415 2187297